Exhibit 99.1

Aytu BioScience Provides First Quarterly Business Update

Live Conference Call and Webcast Today at 4:30 p.m. ET

Englewood, CO – September 28, 2015 – Aytu BioScience, Inc. (OTCQB: AYTU), a specialty healthcare company focused on developing treatments for urological and related conditions, today will host its inaugural quarterly conference call to review recent accomplishments and provide an overview of its business and growth strategy, as well as its financial results for the fiscal year ended June 30, 2015.

Corporate Highlights:

Josh Disbrow, Chief Executive Officer of Aytu BioScience, Inc., stated, “We have executed successfully on several of our business objectives in a relatively short period of time as Aytu BioScience, and think we are poised for growth over the coming quarters. We believe we are well on our way toward becoming a leading specialty healthcare company with a strong footprint and robust pipeline covering high-value areas of urology and related conditions. There will be much more to share as we progress through fiscal 2016 and we look forward to providing the market with additional updates as things develop. We’re excited about what lies ahead for Aytu and are proud of the rapid progress we’ve already made to date.”

The Company successfully merged the pipeline assets of Luoxis Diagnostics and Vyrix Pharmaceuticals with Rosewind Corporation, and subsequently announced its corporate name change to Aytu BioScience, Inc. in June 2015. The name Aytu reflects management’s previous success in building commercial-stage specialty healthcare companies by combining the strengths and experience gained at two previous ‘A’ companies: Arbor Pharmaceuticals, which was founded by Josh and Jarrett Disbrow and built into a revenue-generating enterprise before it was successfully sold; and Ampio Pharmaceuticals, from which two of the Company’s current assets were originally developed.

In May, Aytu acquired ProstaScint^®, a commercial-stage imaging agent used to detect the extent and spread of prostate cancer, from Jazz Pharmaceuticals. ProstaScint^® marks the Company’s first commercial-stage product and its established brand aligns with Aytu’s core business strategy of building a pipeline of late-stage and revenue-generating urology assets. Revenue from ProstaScint^® is expected to help accelerate the growth of the newly formed specialty healthcare company and drive development for its clinical-stage assets. These include Zertane, a first-in-class treatment for premature ejaculation that is nearing commercial-stage, and the RedoxSYS^® System, which has CE Marking for Europe and medical device approval by Health Canada, and its product line extension, which is under development as a newly branded product called MiOXSYS™ for male infertility.

Aytu expects to submit an investigational new drug application (IND) for Zertane^®, which will be followed by the initiation of a first pivotal Zertane study in the U.S. In October, Aytu will present new data at the American Society for Reproductive Medicine’s (ASRM) annual meeting. The data are from a study utilizing MiOXSYS™ to measure oxidative stress levels in semen, which is broadly implicated as a major cause of idiopathic male infertility.

Aytu recently announced that it has begun building the initial commercial infrastructure for FDA-approved ProstaScint^®, as well as its other late-stage product candidates, with the appointment of Jonathan Heath

McGrael as Vice President of Sales, who will lead this commercial effort. Additionally, Aytu has hired a team of highly experienced people in international business development, sales and marketing, clinical development, regulatory and quality affairs, and finance, among others. Aytu plans to hire its initial sales force in the fourth calendar quarter of 2015.

Financial Highlights

Aytu completed a private placement convertible note financing, raising a total of $5.175 million. Together with anticipated revenue from ProstaScint^®, proceeds from the convertible note offering should allow Aytu to initiate a first Phase 3 clinical trial for Zertane^® and advance the MiOXSYS™ System through FDA clinical trials for male infertility.

The Company also announced the registration for resale of 2,564,872 of its common shares that were owned by certain stockholders and issuable to certain warrant holders in Aytu’s predecessor companies. This action was taken to honor Aytu’s registration commitment to the shareholders of its predecessor companies, and to add liquidity to the Company’s stock as it continues to execute on its growth strategy.

Aytu ended its fiscal year 2015 with $7.4 million in cash and cash equivalents, which did not include proceeds from the $5.2 million convertible note offering.

Conference Call Information:

The live conference call and webcast will begin today at 4:30 p.m. Eastern Time. Interested participants and investors may access the conference call by dialing either:

The webcast will be accessible live and archived for 30 days on Aytu’s website, www.aytubio.com.

A replay of the call will be available for seven days beginning at approximately 5:30 p.m., ET on September 28, 2015. Access the replay by calling 1-877-344-7529 (U.S.) or 1-412-317-0088 (international) and using the replay access code 10072642.

About Aytu BioScience, Inc.

Aytu BioScience, Inc. is a specialty healthcare company focused on developing treatments for urological and related conditions. The Company is currently completing Phase 3 clinical development for its lead therapeutic candidate Zertane™, an orally disintegrating tablet (ODT) formulated specifically for the on-demand treatment of premature ejaculation (PE). The Company’s therapeutic pipeline is supported in part by its first-in-class RedoxSYS^® System, which provides the only complete assessment of patients’ redox status and enables identification of a broad variety of disorders where redox is implicated. Clinical studies are underway utilizing a new system developed from the RedoxSYS^® technology called the MiOXSYS™ System in male fertility. The Company also recently acquired and expects to commercialize FDA-approved ProstaScint^® (capromab pendetide), a radio-labeled monoclonal antibody, that targets Prostate Specific Membrane Antigen (PSMA), a protein uniquely expressed by prostate tissue. The Company’s strategy is to develop its core therapeutic and diagnostic assets while building an innovative pipeline of established marketed products and late-stage development assets.

For Investors & Media:

Tiberend Strategic Advisors, Inc.

Joshua Drumm, Ph.D.: jdrumm@tiberend.com; (212) 375-2664

Janine McCargo: jmccargo@tiberend.com; (646) 604-5150

Forward Looking Statement

This press release includes forward-looking statements within the meaning of Section 27A of the Securities Act of 1933, as amended, and Section 21E of the Securities Exchange Act of 1934, or the Exchange Act. All statements other than statements of historical facts contained in this presentation, including statements regarding our anticipated future clinical and regulatory events, future financial position, business strategy and plans and objectives of management for future operations, are forward-looking statements. Forward looking statements are generally written in the future tense and/or are preceded by words such as “may,” “will,” “should,” “forecast,” “could,” “expect,” “suggest,” “believe,” “estimate,” “continue,” “anticipate,” “intend,” “plan,” or similar words, or the negatives of such terms or other variations on such terms or comparable terminology. Such forward-looking statements include, without limitation, statements regarding the potential future development and commercialization of our product candidates, the anticipated start dates, durations and completion dates, costs, as well as the potential future results, of our ongoing and future clinical trials, the anticipated designs of our future clinical trials, anticipated future regulatory submissions and events, our anticipated future cash position and future events under our current and potential future collaborations. These forward-looking statements are subject to a number of risks, uncertainties and assumptions, including without limitation to the risks described in “Risk Factors” in the Company’s Annual Report on Form 10-K filed on September 28, 2015 and in the other reports and documents we file with the Securities and Exchange Commission from time to time. Moreover, we operate in a very competitive and rapidly changing environment. New risk factors emerge from time to time and it is not possible for our management to predict all risk factors, nor can we assess the impact of all factors on our business or the extent to which any factor, or combination of factors, may cause actual results to differ materially from those contained in any forward-looking statements. You should not rely upon forward-looking statements as predictions of future events. We cannot assure you that the events and circumstances reflected in the forward-looking statements will be achieved or occur and actual results could differ materially from those projected in the forward-looking statements. We assume no obligation to update or supplement forward-looking statements.

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Balance Sheet

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Statements of Operations

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Statements of Cash Flows