27 April 2021 First Quarter 2021 Earnings Call Presentation

Forward-Looking Statements and Non-GAAP Financial Measures 1 Forward-looking statements in this report relating to WesBanco’s plans, strategies, objectives, expectations, intentions and adequacy of resources, are made pursuant to the safe harbor provisions of the Private Securities Litigation Reform Act of 1995. The information contained in this report should be read in conjunction with WesBanco’s Form 10-K for the year ended December 31, 2020 and documents subsequently filed by WesBanco with the Securities and Exchange Commission (“SEC”), which are available at the SEC’s website, www.sec.gov or at WesBanco’s website, www.WesBanco.com. Investors are cautioned that forward-looking statements, which are not historical fact, involve risks and uncertainties, including those detailed in WesBanco’s most recent Annual Report on Form 10-K filed with the SEC under “Risk Factors” in Part I, Item 1A. Such statements are subject to important factors that could cause actual results to differ materially from those contemplated by such statements, including, without limitation, the effects of changing regional and national economic conditions including the effects of the COVID-19 pandemic; changes in interest rates, spreads on earning assets and interest-bearing liabilities, and associated interest rate sensitivity; sources of liquidity available to WesBanco and its related subsidiary operations; potential future credit losses and the credit risk of commercial, real estate, and consumer loan customers and their borrowing activities; actions of the Federal Reserve Board, the Federal Deposit Insurance Corporation, the SEC, the Financial Institution Regulatory Authority, the Municipal Securities Rulemaking Board, the Securities Investors Protection Corporation, and other regulatory bodies; potential legislative and federal and state regulatory actions and reform, including, without limitation, the impact of the implementation of the Dodd-Frank Act; adverse decisions of federal and state courts; fraud, scams and schemes of third parties; cyber-security breaches; competitive conditions in the financial services industry; rapidly changing technology affecting financial services; marketability of debt instruments and corresponding impact on fair value adjustments; and/or other external developments materially impacting WesBanco’s operational and financial performance. WesBanco does not assume any duty to update forward-looking statements. In addition to the results of operations presented in accordance with Generally Accepted Accounting Principles (GAAP), WesBanco's management uses, and this presentation contains or references, certain non-GAAP financial measures, such as pre-tax pre-provision income, tangible common equity/tangible assets; net income excluding after-tax restructuring and merger-related expenses; efficiency ratio; return on average assets; and return on average tangible equity. WesBanco believes these financial measures provide information useful to investors in understanding our operational performance and business and performance trends which facilitate comparisons with the performance of others in the financial services industry. Although WesBanco believes that these non-GAAP financial measures enhance investors' understanding of WesBanco's business and performance, these non-GAAP financial measures should not be considered an alternative to GAAP. The non-GAAP financial measures contained therein should be read in conjunction with the audited financial statements and analysis as presented in the Annual Report on Form 10-K as well as the unaudited financial statements and analyses as presented in the Quarterly Reports on Forms 10-Q for WesBanco and its subsidiaries, as well as other filings that the company has made with the SEC.

Q1 2021 Financial and Operational Highlights 2 Note: financial and operational highlights during the quarter ended March 31, 2021; loan growth includes approximately $824 million of loans funded through the Small Business Administration’s Paycheck Protection Program (“SBA PPP”), as established by the CARES Act (1) Non-GAAP measure – please see reconciliation in appendix Strong growth in pre-tax, pre-provision income Continued emphasis on expense management Improving macro-economic factors drove a $28 million release of provision for credit losses Key credit quality metrics remained at low levels and favorable to peer bank averages Positive growth in both loans and deposits Mortgage banking income increased due to a high volume of originations WesBanco is well-capitalized with solid liquidity and a strong balance sheet Recent Board authorized stock repurchase program, when combined with the remainder of the previous authorization, represents approximately 5% of outstanding shares Pre-Tax, Pre-Provision Income(1) $64.2 million, +3.6% YoY Net Income Available to Common Shareholders and Diluted EPS(1) $71.3 million; $1.06/diluted share Efficiency Ratio(1) 56.71% Mortgage Banking Income $4.3 million, +234.2% YoY Loan Growth +3.4% YoY Deposit Growth (x-CDs) +28.9% YoY

Q1 2021 Key Metrics 3 Note: PTPP = pre-tax, pre-provision Non-GAAP measure – please see reconciliation in appendix Excludes restructuring and merger-related expenses

Q1 2021 Total Portfolio Loans ($MM) 4 ~7,750 SBA PPP loans totaling ~$824 million (as of 3/31/2021) During Q1 2021, ~2,330 customers applied for and received forgiveness of their 2020 SBA PPP loans totaling $223 million; and, assisted >3,240 businesses with 2021 SBA PPP loans totaling ~$344 million C&I loan levels (x-SBA PPP) were down year-over-year primarily due to lower utilization of revolving lines of credit (~33% vs. ~43% last year) Q1 2021 residential real estate loan levels impacted by retaining ~40% of the $326 million of origination dollar volume (~57% refi) on balance sheet Home equity and consumer loan levels negatively impacted by payoffs driven by utilization of residential mortgage refinancing and higher personal savings

Q1 2021 Net Interest Margin (NIM) 5 NIM negatively impacted by the low interest rate environment As a result of higher cash balances, investment securities increased by $0.9 billion during Q1 2021, mostly during March Aggressively reduced deposit rates throughout the past year Q1 2021 interest-bearing deposit funding costs 20bp, or, when including non-interest bearing deposits, 14bp Period-end FHLB borrowings of $0.4 billion, with remaining average life of less than one year, down $1.2 billion year-over-year SBA PPP loans benefited Q1 2021 NIM by a net 11bp, mainly due to 2020 SBA PPP originations forgiven Note: commercial loan portfolio index mix excludes SBA PPP loans

Q1 2021 Non-Interest Income 6 Mortgage banking fees increased due to an ~50% year-over-year increase in 1-to-4 family residential mortgage origination dollar volume, and the associated sale of ~60% of those into the secondary market Trust fees increased due to equity market improvement and organic growth in trust assets Other income increased due to higher loan swap-related income, which was primarily the result of $2.8 million of fair market value adjustments in the current period as compared to a negative $2.8 million adjustment last year Service charges on deposits were lower due to higher consumer deposits associated with the three rounds of stimulus to-date and lower general consumer spending, resulting in fewer eligible account fees Note: OREO = other real estate owned

Q1 2021 Non-Interest Expense 7 Total operating expenses remained well-controlled through company-wide efforts to manage open positions and certain discretionary expenses Efficiency ratio improved 98bp year-over-year to 56.71% Lower salaries and wages reflect the recent financial center closures and the management of FTEs Anticipated gross cost savings of ~$6 million from closures remain on track to be fully realized during Q2 2021 Marketing expense was higher due to increased product advertising and brand awareness campaigns that were delayed from 2020 due to the COVID-19 pandemic Q1 restructuring & merger-related charges related to the financial center optimization plan that was completed during January 2021

Non-Performing Assets as % of Total Assets Net Charge-Offs as % of Average Loans (Annualized) Allowance for Credit Losses as % of Total Loans Criticized & Classified Loans as % of Total Loans Favorable asset quality measures compared to all U.S. banks with total assets from $10B to $25B Strong Legacy of Credit Quality 8 Note: financial data as of quarter ending for dates specified; Current Expected Credit Losses (“CECL”) accounting standard adopted January 1, 2020 by WSBC; peer bank group includes all U.S. banks with total assets of $10B to $25B; peer data from S&P Global Market Intelligence (as of 4/16/2021) and represent simple averages

The decrease in the allowance was driven by improvement in the macroeconomic forecast and changes in portfolio mix slightly offset by COVID-19 pandemic related adjustments Allowance coverage ratio of 1.50%, or, excluding SBA PPP loans, 1.62% Excludes fair market value adjustments on previously acquired loans representing 0.34% of total portfolio loans Q1 2021 Current Expected Credit Loss (CECL) 9 Note: ACL at 3/31/2021 excludes off-balance sheet credit exposures of $6.7 million; on January 1, 2020, WSBC adopted the CECL accounting standard (prior to this date, the allowance for credit losses was calculated under the incurred method)

Strong regulatory capital ratios significantly above both regulatory requirements and well-capitalized levels; and, higher tangible equity levels New stock repurchase plan of up to 1.7 million shares from time to time on the open market, combined with ~1.7 million shares remaining under the previous authorization, represents ~5% of outstanding shares (1) Tangible Equity to Tangible Assets (1) Tier 1 Risk-Based Capital Ratio 10 Strong Capital Position Note: financial data as of quarter ending 12/31; current year data as of 3/31/2021; CECL accounting standard adopted January 1, 2020 by WSBC; peer bank group as of 12/31/20 includes all U.S. banks with total assets of $10B to $25B from S&P Global Market Intelligence (as of 4/16/2021) and represent simple averages New authorization approved by Board of Directors on April 22, 2021, and prior authorization approved on December 19, 2019 Non-GAAP measure – please see reconciliation in appendix Well-Capitalized 8.0% Required 6.0% Peers 13.66% Peers 9.85%

Appendix

Reconciliation: Pre-Tax, Pre-Provision Income (PTPP) and Ratios 12

Reconciliation: Net Income, EPS (Diluted), Tangible Book Value per Share 13

Reconciliation: Efficiency Ratio 14 Note: “efficiency ratio” is non-interest expense excluding restructuring and merger-related expense divided by total income; FTE represents fully taxable equivalent

Reconciliation: Return on Average Assets 15 (1) three-, six-, and nine-month (as applicable) figures are annualized

Reconciliation: Return on Average Tangible Equity 16 (1) three-, six-, and nine-month (as applicable) figures are annualized

Reconciliation: Tangible Equity to Tangible Assets 17 Note: Old Line Bancshares merger closed November 2019; Farmers Capital Bank Corporation merger closed August 2018; First Sentry Bancshares merger closed April 2018; Your Community Bankshares merger closed September 2016; ESB Financial merger closed February 2015; Fidelity Bancorp merger closed November 2012; AmTrust 5 branch acquisition closed March 2009; Oak Hill Financial closed November 2007