Exhibit 10.1

7700 Irvine Center Drive, Suite 700

Irvine, CA 92618

January 13, 2017

Mr. Vladimir Feingold

c/o PresbiBio, LLC

7700 Irvine Center Drive, Suite 700

Irvine, CA 92618

Dear Vlad:

Further to our on-going discussions of a potential transition from an executive officer role to a more limited consulting role, while continuing to serve on the board of directors of Presbia PLC, we mutually agree that your employment with PresbiBio, LLC, a subsidiary of Presbia PLC (hereafter “Company”) ended effective as of January 13, 2017. This Separation and General Release Agreement (the “Agreement”) confirms the terms of the separation of your employment, including the payment and consulting opportunity described in paragraph 3 below that you will receive if you sign and return this Agreement to the Company no later than 21 days from today and do not revoke this Agreement during the 7-day revocation period described below in paragraph 6.

By signing, delivering and not revoking this Agreement, you will be entering into a binding agreement with the Company and will be agreeing to the terms and conditions in the numbered paragraphs below, including the general release of claims in paragraphs 4, 5, and 6. Therefore, you are advised to consult with an attorney of your choice before signing this Agreement. If you choose not to sign and return this Agreement within the required time-period or if you revoke your acceptance of this Agreement, you will not receive the payment or consulting opportunity described in paragraph 3.

1. Last Day of Employment. You acknowledge that you resigned from your employment with the Company as of January 13, 2017 (the “Separation Date”). You shall remain on the Company’s health insurance plan through January 13, 2017, at which time you will be eligible for COBRA at your own expense. Information about COBRA will follow under separate cover. Participation in all other Company benefits ends as of the Separation Date.

2. Final Salary/Wages; Vacation Pay. You have received your final pay check as of the Separation Date, which includes payment for all salary/wages that you earned through the Separation Date, less applicable withholdings and deductions. All unused and accrued vacation shall be paid to you upon execution of this Agreement. You will receive these payments even if you do not enter into this Agreement.

3. Consideration. If you choose to sign and return this Agreement within the required time-period, do not revoke this Agreement and abide by the other terms of this Agreement, the Company agrees to provide you with the following: (a) 25% of your available bonus for achievement of your personal development goals for 2016, which equates to not less than $20,472; (b) 25% of your available bonus which may range from $0 to $20,472 if, and to the extent that, the Company achieved its corporate goals for 2016 as set

forth in the Company’s internal bonus plan measured in the first quarter of 2017, such sum, if any, to be paid to you by March 31, 2017, and to be calculated and paid consistent with the bonus treatment of other of the Company’s senior executives; and (c) the consulting opportunity available to you as set forth in the annexed Independent Contractor Services Agreement (the “Consulting Agreement”). You acknowledge that you are not otherwise entitled to this payment or consulting opportunity and that the Company would not agree to provide you with the payment or consulting opportunity as set forth in the annexed Consulting Agreement without your general release of claims and other promises in this Agreement. You agree that the terms of the Consulting Agreement constitute good and valuable consideration for your general release of claims and other promises in this Agreement.

4. General Release of Claims. In exchange for the consideration described in paragraph 3 above to which you are not otherwise entitled, you (for yourself and your heirs, executors, administrators, beneficiaries, personal representatives and assigns) hereby completely, forever, irrevocably and unconditionally release and discharge, to the maximum extent permitted by law, the Company, the Company’s past, present and future parent organizations, subsidiaries, affiliated entities, managers, related companies and divisions and each of their respective past, present and future officers, directors, employees, shareholders, trustees, members, partners, attorneys and agents (in each case, individually and in their official capacities) and each of their respective employee benefit plans (and such plans’ fiduciaries, agents, administrators and insurers, individually and in their official capacities), as well as any predecessors, future successors or assigns or estates of any of the foregoing (the “Released Parties”) from any and all claims, actions, charges, controversies, causes of action, suits, rights, demands, liabilities, obligations, damages, costs, expenses, attorneys’ fees, damages, and obligations of any kind or character whatsoever, that you ever had, now have or may in the future claim to have by reason of any act, conduct, omission, transaction, agreement, occurrence or any other matter whatsoever occurring up to and including the date you sign this Agreement. This general release of claims includes, without limitation, any and all claims:

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You expressly acknowledge that this general release of claims includes any and all claims arising up to and including the date you sign this Agreement which you have or may have against any of the Released Parties, whether such claims are known or unknown, suspected or unsuspected, asserted or unasserted, disclosed or undisclosed. By signing this Agreement, you expressly waive any right to assert that any such claim, demand, obligation or cause of action has, through ignorance or oversight, been omitted from the scope of this release and you further waive any rights under statute or common law principles that otherwise prohibits the release of unknown claims.

This general release of claims does not apply to, waive or affect: any rights or claims that may arise after the date you sign this Agreement; any claim for workers’ compensation benefits (but it does apply to, waive and affect claims of discrimination and/or retaliation on the basis of workers’ compensation status); claims for unemployment benefits; claims for indemnification in connection with your service as an officer and/or director; or any other claims or rights that by law cannot be waived in a private agreement between an employer and employee; or your rights to any vested benefits to which you are entitled under the terms of the applicable employee benefit plan (the “Excluded Claims”). This general release of claims also does not apply to, waive, affect, limit or interfere with your rights under paragraph 11 below.

5. Further Release By You Of the Released Parties. You expressly acknowledge that, in further consideration of the payment and consulting opportunity set forth in paragraph 3, you waive all rights afforded by Section 1542 of the Civil Code of the State of California (“Section 1542”), or any other law or statute of similar effect in any jurisdiction with respect to the released Claims, with respect to the Released Parties. Section 1542 states: “A GENERAL RELEASE DOES NOT EXTEND TO CLAIMS WHICH THE CREDITOR DOES NOT KNOW OR SUSPECT TO EXIST IN HIS OR HER FAVOR AT THE TIME OF EXECUTING THE RELEASE, WHICH IF KNOWN BY HIM OR HER MUST HAVE MATERIALLY AFFECTED HIS OR HER SETTLEMENT WITH THE DEBTOR.” Notwithstanding the provisions of Section 1542 and for the purpose of implementing a full and complete release of all Claims, you expressly acknowledge and agree that this Agreement releases all Claims existing or arising prior to your execution of this Agreement which you have or suspect you may have against the Released Parties whether such claims are known or unknown and suspected or unsuspected by you and you forever waive all inquiries and investigations into any and all such claims. You understand and acknowledge that the significance and consequence of this waiver of Civil Code §1542, is that even if you should suffer additional injuries or damages arising out of the released Claims, you will not be permitted to make any claim for those injuries or damages.

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6. Waiver of Claims under ADEA; Time to Consider/Revoke. You acknowledge, understand and agree that that the general release of claims in paragraphs 4 and 5 above includes, but is not limited to, a waiver and release of all claims that you may have under the Age Discrimination in Employment Act of 1967, as amended (the “ADEA”) arising up to and including the date that you sign this Agreement. As required by the Older Workers Benefit Protection Act of 1990, you are hereby advised that:

7. No Pending Claims. You represent and warrant that you have no charges, lawsuits, or actions pending in your name against any of the Released Parties relating to any claim that has been released in this Agreement. You also represent and warrant that you have not assigned or transferred to any third party any right or claim against any of the Released Parties that you have released in this Agreement.

8. Covenant not to Sue. Except as provided in paragraph 11 below, you covenant and agree that you will not report, institute or file a charge, lawsuit or action (or encourage, solicit, or voluntarily assist or participate in, the reporting, instituting, filing or prosecution of a charge, lawsuit or action by a third party) against any of the Released Parties with respect to any claim that has been released in this Agreement.

9. Cooperation with Investigations/Litigation. You agree, at the Company’s request, to reasonably cooperate, by providing truthful information, documents and testimony, in any Company investigation, litigation, arbitration, or regulatory proceeding regarding events that occurred during your employment with the Company. Your requested cooperation may include, for example, making yourself reasonably available to consult with the Company’s counsel, providing truthful information and documents, and to appear to give truthful testimony. The Company will, to the extent permitted by applicable law and court rules, reimburse you for reasonable out-of-pocket expenses that you incur in providing any requested cooperation, so long as you provide advance written notice to the Company of your request for reimbursement and provide satisfactory documentation of your expenses. Following the later of the

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termination of the Consulting Agreement or your tenure as a director, the Company will also compensate you for your time to the extent permitted by applicable law and court rules, at a mutually agreed upon rate. Nothing in this paragraph is intended to, and shall not, preclude or limit your protected rights under paragraph 11 below or restrict or limit you from providing truthful information in response to a subpoena, other legal process or valid governmental inquiry.

10. Non-Disparagement. The Company’s Officers and Directors, on the one hand, and you, on the other hand, hereby agree not to at any time make any disparaging or derogatory statements concerning each other, or the Company’s business, products and services. However, nothing in this paragraph is intended to, and shall not, restrict or limit you from (a) exercising your protected rights described in paragraph 11, or (b) providing truthful information in response to a subpoena, other legal process or valid governmental inquiry. The Company agrees to instruct its officers and directors not to make any disparaging or derogatory statements concerning you.

11. Fiduciary Duties; Corporate Governance; Restricted Stock. You acknowledge that the termination of your employment has no impact on your continuing fiduciary duties to the Company, stemming from your continued service on the Company’s board of directors. You further acknowledge that as long as you are a member of the board you continue to be subject to the Company’s code of business conduct, insider trading policy and other corporate governance policies in the same manner as any member of the board of directors. Provided that there is an open window under the Company’s insider trading policy at the time of such request, the Company hereby agrees to instruct its counsel to issue opinions to remove restrictive legends from shares held by you upon presentation by you of selling stockholder representation letters and broker representation letters, each in a form reasonably satisfactory to such counsel, that such shares are eligible to be sold under Rule 144 of the Securities Act of 1933, as amended (“Rule 144”). In addition, the Company hereby agrees that ninety (90) days after your resignation from the Board of Directors of the Company, it will instruct its counsel to issue opinions to remove all restrictive legends from shares held by you for at least one year, upon presentation by you of selling stockholder representation letter(s), each in a form reasonably satisfactory to such counsel, certifying that (i) you are not, and have not been within the past three months, an affiliate of the Company and (ii) you have held the shares for at least one year, so that such shares are eligible to be sold under Rule 144 without any restrictions.

12. Acknowledgments. You and the Company acknowledge and agree that:

(A) By entering in this Agreement, you do not waive any rights or claims that may arise after the date that you sign and deliver this Agreement to the Company;

(B) This Agreement is not intended to, and shall not in any way prohibit, limit or otherwise interfere with your protected rights under federal, state or local law to without notice to the Company: (i) communicate or file a charge with a government regulator; (ii) participate in an investigation or proceeding conducted by a government regulator; or (iii) receive an award paid by a government regulator for providing information;

(C) Notwithstanding anything set forth in this Agreement to the contrary, nothing in this Agreement shall affect or be used to interfere with your protected right to test in any court, under the Older Workers’ Benefit Protection Act, or like statute or regulation, the validity of the waiver of rights under ADEA as set forth in this Agreement; and

(D) Nothing in this Agreement shall preclude you from exercising your rights, if any (i) under Section 601-608 of the Employee Retirement Income Security Act of 1974, as amended, popularly known as COBRA, or (ii) the Company’s 401(k) plan.

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13. No Other Pay or Benefits. You acknowledge and agree that upon the Company’s payment of the amounts described in paragraph 2, you will have been paid for all work performed including, without limitation, all salary/wages, bonuses, overtime, commissions and any earned, but unused, vacation time due to you up through and including the last day of your employment. You acknowledge and agree that, except for Company’s obligation to provide the payment and consulting opportunity provided in paragraph 3, you are entitled to no other payments or benefits whatsoever (other than COBRA) and the Released Parties have no further obligations to you whatsoever, whether arising out of your employment with the Company, your separation from the Company or otherwise.

14. No Admission. Nothing contained in this Agreement will constitute or be treated as an admission by you, the Company or any of the other Released Parties of any liability, wrongdoing or violation of law.

15. Miscellaneous.

(A) This Agreement, together with the Employee Non-Disclosure and Invention Assignment Agreement (referred to herein as the “Covenants Agreement”), contains the entire agreement and understanding between you and the Company concerning the subject matter of this Agreement and supersedes any and all prior agreements or understandings (both written and oral) between you and the Company concerning the subject matter of this Agreement. This Agreement may only be modified by a written document signed by you and an authorized officer of the Company.

(B) This Agreement and the Covenants Agreement shall inure to the benefit of the Company and the other Released Parties and shall be binding upon Company and its successors and assigns. This Agreement also shall inure to the benefit of, and be binding upon, you and your heirs, executors, administrators, beneficiaries, personal representatives and assigns. This Agreement is personal to you and you may not assign or delegate your rights or duties under this Agreement, and any such assignment or delegation will be null and void.

(C) The provisions of this Agreement and the Covenants Agreement are severable. If any provision in this Agreement or the Covenants Agreement is held to be invalid, illegal or unenforceable, the remaining provisions will remain in full force and effect and the invalid, illegal and unenforceable provision shall be reformed and construed so that it will be valid, legal and enforceable to the maximum extent permitted by law.

(D) The Company and you shall each bear their own costs, fees (including, without limitation, attorney’s fees) and expenses in connection with the negotiation, preparation and execution of this Agreement.

(E) The failure of the Company to seek enforcement of any provision of this Agreement or the Covenants Agreement in any instance or for any period of time shall not be construed as a waiver of such provision or of the Company’s right to seek enforcement of such provision in the future.

(F) This Agreement and the Covenants Agreement will be governed and interpreted under the laws of the State of California, without giving effect to choice of law principles. The Company and you irrevocably consent to the exclusive jurisdiction of the federal and state courts in the State of California for the resolution of any disputes arising under or respect to this Agreement or the Covenants Agreement.

(G) Given the full and fair opportunity provided to each of party to consult with their respective counsel regarding terms of this Agreement, ambiguities shall not be construed against either party by virtue of such party having drafted the subject provision.

(H) The headings in this Agreement are included for convenience of reference only and shall not affect the interpretation of this Agreement.

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16. Opportunity to Review. You represent and warrant that you:

If you wish to accept this Agreement, please sign, date and return it no later than twenty-one (21) days after you receive it.

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