Subsidiaries of Hologic

Jurisdiction of Incorporation or Organization

AEG Elektrofotografie GmbH

Germany

AEG Photoconductor (Shanghai) Co. Ltd.

China

AEG Photoconductor Corporation

Delaware

BioLucent, LLC

Delaware

Cruiser, Inc.

Delaware

Hologic (Australia) PTY Ltd.

Australia

Hologic Canada, Ltd

Canada

Cytyc Cayman Limited

Cayman Islands

Cytyc Corporation

Delaware

Cytyc Development Company, LLC

Delaware

Hologic Benelux B.V.

Netherlands

Hologic Europe Middle East and Africa, S.A.

Switzerland

Hologic France, Sarl

France

Hologic Deutschland GmbH

Germany

Hologic Asia Pacific Limited

Hong Kong

Hologic Iberia (Spain), SL

Spain

Cytyc Interim, Inc.

Delaware

Cytyc International, Inc.

Delaware

Hologic Italia S.r.l.

Italy

Cytyc S.a.r.l.

Switzerland

Cytyc Limited Liability Company

Delaware

Cytyc Prenatal Products Corp.

Delaware

Cytyc Securities Corporation

Massachusetts

Hologic Suisse, S.A.

Switzerland

Cytyc Surgical Products II, Limited Partnership.

Massachusetts

Cytyc Surgical Products III, Inc.

Delaware

Cytyc Surgical Products Limited Partnership

Massachusetts

Direct Radiography Corp.

Delaware

Hologic Asia Limited

Hong Kong

Hologic Espana S.A.

Spain

Hologic N.V.

Belgium

Hologic France S.A.

France

Hologic International Holdings B.V.

Netherlands

Hologic Investment Corp.

Massachusetts

Hologic Limited Partnership

Massachusetts

Hologic Surgical Products Costa Rica, S.A.

Costa Rica

Hologic (UK) Limited

England and Whales

R2 Technology Canada, Inc.

Ontario

R2 Technology, Inc.

Delaware

SST Merger Corp.

Delaware

Suros Surgical Systems, Inc.

Delaware

Third Wave Technologies, Inc.

Delaware

Third Wave Agbio Inc.

Delaware

Third Wave Japan, Inc.

Japan